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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP6 Polyclonal antibody (19163-1-AP)

    WB analysis using 19163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19163-1-AP (FKBP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FKBP6 Polyclonal antibody (19163-1-AP)

    IF Staining of MCF-7 using 19163-1-AP

    Immunofluorescent analysis of MCF-7 cells, using FKBP6 antibody 19163-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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FKBP6 Polyclonal antibody

FKBP6 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19163-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

36 kDa FK506 binding protein, 36 kDa FKBP, FK506 binding protein 6, FK506 binding protein 6, 36kDa, FKBP 36, FKBP 6, FKBP36, FKBP6, Immunophilin FKBP36, PPIase, PPIase FKBP6, Rotamase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat testis tissue, human testis tissue, mouse testis tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19163-1-AP targets FKBP6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP6 fusion protein Ag4642 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 6, 36kDa
Calculated molecular weight 327 aa, 37 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC036817
Gene Symbol FKBP6
Gene ID (NCBI) 8468
RRIDAB_10603363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75344
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Inactive peptidyl-prolyl cis-trans isomerase FKBP6 (FKBP6), also known as 36 kDa FK506-binding protein (36 kDa FKBP; FKBP-36), is a member of a gene family that contains a prolyl isomerase/FK506 binding domain and tetratricopeptide protein-protein interaction domains(PMID: 9782077). It has been linked to the secondary piRNA biogenesis in fetal male mouse germ cells ( PMID: 22902560).

Protocols

Product Specific Protocols
WB protocol for FKBP6 antibody 19163-1-APDownload protocol
IF protocol for FKBP6 antibody 19163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols