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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FKBP7 Polyclonal antibody

FKBP7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12092-1-AP

Synonyms

23 kDa FK506 binding protein, 23 kDa FKBP, FK506 binding protein 7, FKBP 23, FKBP 7, FKBP23, FKBP7, PPIase, PPIase FKBP7, Rotamase

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using FKBP7 Polyclonal antibody (12092-1-AP)

    WB analysis of human lung using 12092-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12092-1-AP (FKBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using FKBP7 Polyclonal antibody (12092-1-AP)

    WB analysis of human heart using 12092-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12092-1-AP (FKBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FKBP7 Polyclonal antibody (12092-1-AP)

    IHC staining of human liver cancer using 12092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12092-1-AP (FKBP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FKBP7 Polyclonal antibody (12092-1-AP)

    IHC staining of human liver cancer using 12092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12092-1-AP (FKBP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FKBP7 Polyclonal antibody (12092-1-AP)

    IHC staining of human lung cancer using 12092-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12092-1-AP (FKBP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FKBP7 Polyclonal antibody (12092-1-AP)

    IHC staining of human lung cancer using 12092-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12092-1-AP (FKBP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman lung tissue, human heart tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12092-1-AP targets FKBP7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP7 fusion protein Ag2733 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 7
Calculated molecular weight 259 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009711
Gene symbol FKBP7
Gene ID (NCBI) 51661
RRIDAB_2103418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP7(FK506-binding protein 7) is also named as FKBP23 and belongs to immunophilin class of proteins family. This protein contains an N-terminal hydrophobic signal sequence, a PPIase motif, 2 EF-hand domains, and 2 putative N-glycosylation sites. The calculated molecular mass is about 25 kD, but becomes 33 kD following cleavage of the signal sequence(PMID:9806833). It could bind to BiP in ER in a process regulated by the concentration of Ca2+(PMID:22269648). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FKBP7 antibody 12092-1-APDownload protocol
IHC protocol for FKBP7 antibody 12092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols