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FKBP7 Polyclonal antibody

FKBP7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12092-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

23 kDa FK506 binding protein, 23 kDa FKBP, FK506 binding protein 7, FKBP 23, FKBP 7, FKBP23, FKBP7, PPIase, PPIase FKBP7, Rotamase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 700 μg/ml
20 μL, 700 μg/ml
150 μL, 700 μg/ml

¥67,000/ 150 μL

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Tested Applications

Positive WB detected inhuman lung tissue, human heart tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12092-1-AP targets FKBP7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP7 fusion protein Ag2733 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 7
Calculated molecular weight 259 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009711
Gene Symbol FKBP7
Gene ID (NCBI) 51661
RRIDAB_2103418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP7(FK506-binding protein 7) is also named as FKBP23 and belongs to immunophilin class of proteins family. This protein contains an N-terminal hydrophobic signal sequence, a PPIase motif, 2 EF-hand domains, and 2 putative N-glycosylation sites. The calculated molecular mass is about 25 kD, but becomes 33 kD following cleavage of the signal sequence(PMID:9806833). It could bind to BiP in ER in a process regulated by the concentration of Ca2+(PMID:22269648). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FKBP7 antibody 12092-1-APDownload protocol
IHC protocol for FKBP7 antibody 12092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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