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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using FKBP9 Polyclonal antibody (18073-1-AP)

    WB analysis of NIH/3T3 using 18073-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18073-1-AP (FKBP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using FKBP9 Polyclonal antibody (18073-1-AP)

    WB analysis of L02 using 18073-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 18073-1-AP (FKBP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using FKBP9 Polyclonal antibody (18073-1-AP)

    IF Staining of L02 using 18073-1-AP

    Immunofluorescent analysis of L02 cells using 18073-1-AP (FKBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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FKBP9 Polyclonal antibody

FKBP9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18073-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FK506-binding protein 9, FK506 binding protein 9, EC:5.2.1.8, 63 kDa FKBP, 63 kDa FK506-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, L02 cells
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18073-1-AP targets FKBP9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP9 fusion protein Ag12715 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 9, 63 kDa
Calculated molecular weight 570 aa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC101723
Gene Symbol FKBP9
Gene ID (NCBI) 11328
RRIDAB_2878490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95302
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP9 (FK506-binding protein 9, also referred to as FKBP60 or FKBP63) belongs to a family of immunophilins that are able to bind to FK506, an immunosuppressive drug. Several members of the FKBP family, including FKBP9, FKBP13, FKBP23 and FKBP65, localize to the endoplasmic reticulum (ER) as they contain the ER retention motif H/R/KDEL (PMID:32111229). In addition, the FKBP9 mutation was also reported to be associated with disease-free survival rates in pheochromocytoma or paraganglioma (PMID:28386672).

Protocols

Product Specific Protocols
WB protocol for FKBP9 antibody 18073-1-APDownload protocol
IF protocol for FKBP9 antibody 18073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genes Dis

The stability of FKBP9 maintained by BiP is crucial for glioma progression

Authors - Shirong Li
View Article