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KD/KO Validated

FKBPL Polyclonal antibody

Name: FKBPL Polyclonal antibody
Brand: Proteintech
SKU: 10060-1-AP

FKBPL Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC (Intra), IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10060-1-AP

Synonyms

WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1

Validation Data Gallery

Filter:

AllWBIPIHCFC (Intra)

WB analysis of HEK-293 using 10060-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10060-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10060-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 10060-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10060-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10060-1-AP
IP result of anti-FKBPL (IP:10060-1-AP, 3ug; Detection:10060-1-AP 1:800) with MCF-7 cells lysate 2500ug.
IHC staining of human breast cancer using 10060-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10060-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 10060-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 10060-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 10060-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Polyclonal antibody (10060-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10060-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, human brain tissue, human testis tissue, Jurkat cells, MCF-7 cells, T-47D cells, MDA-MB-453s cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 19 publications below
IHC	See 7 publications below
IF	See 8 publications below
ChIP	See 1 publications below

Product Information

10060-1-AP targets FKBPL in WB, IHC, IF, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FKBPL fusion protein Ag0112 相同性解析による交差性が予測される生物種
Full Name	FK506 binding protein like
Calculated molecular weight	349 aa, 38 kDa
Observed molecular weight	42-48 kDa
GenBank accession number	BC004168
Gene symbol	FKBPL
Gene ID (NCBI)	63943
RRID	AB_2262677
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193). It can be detected the band between 38 kDa and 48 kDa by western blot.

Protocols

Product Specific Protocols
WB protocol for FKBPL antibody 10060-1-AP	Download protocol
IHC protocol for FKBPL antibody 10060-1-AP	Download protocol
IP protocol for FKBPL antibody 10060-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IF	J Adv Res Hsa_circ_0001402 alleviates vascular neointimal hyperplasia through a miR-183-5p-dependent regulation of vascular smooth muscle cell proliferation, migration, and autophagy Authors - Jia-Jie Lin View Article
human	WB	Cancer Res FKBPL regulates estrogen receptor signaling and determines response to endocrine therapy. Authors - McKeen Hayley D HD View Article
human	IF	Cell Mol Life Sci A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia Authors - Sahar Masoumeh Ghorbanpour View Article
human	WB	J Control Release Delivery of RALA/siFKBPL nanoparticles via electrospun bilayer nanofibres: An innovative angiogenic therapy for wound repair. Authors - Eoghan J Mulholland KD Validated View Article
human	WB,IHC	Clin Cancer Res FKBPL and peptide derivatives: novel biological agents that inhibit angiogenesis by a CD44-dependent mechanism. Authors - Valentine Andrea A View Article
human	WB, ChIP	Oncogene Identification of RBCK1 as a novel regulator of FKBPL: implications for tumor growth and response to tamoxifen. Authors - C Donley View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FKBPL Polyclonal antibody

FKBPL Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 10060-1-AP

WB analysis of human brain using 10060-1-AP

WB analysis of Jurkat using 10060-1-AP

WB analysis of human testis using 10060-1-AP

WB analysis of HeLa using 10060-1-AP

IP experiment of MCF-7 using 10060-1-AP

IHC staining of human breast cancer using 10060-1-AP

IHC staining of human breast cancer using 10060-1-AP

IHC staining of human testis using 10060-1-AP

IHC staining of human testis using 10060-1-AP

FC experiment of HeLa using 10060-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hsa_circ_0001402 alleviates vascular neointimal hyperplasia through a miR-183-5p-dependent regulation of vascular smooth muscle cell proliferation, migration, and autophagy

FKBPL regulates estrogen receptor signaling and determines response to endocrine therapy.

A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia

Delivery of RALA/siFKBPL nanoparticles via electrospun bilayer nanofibres: An innovative angiogenic therapy for wound repair.

FKBPL and peptide derivatives: novel biological agents that inhibit angiogenesis by a CD44-dependent mechanism.

Identification of RBCK1 as a novel regulator of FKBPL: implications for tumor growth and response to tamoxifen.