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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FKBPL Monoclonal antibody

FKBPL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2H10B10

Cat no : 66389-1-Ig

Synonyms

WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FKBPL Monoclonal antibody (66389-1-Ig)

    WB analysis using 66389-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FKBPL Monoclonal antibody (66389-1-Ig)

    WB analysis using 66389-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66389-1-Ig (FKBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FKBPL Monoclonal antibody (66389-1-Ig)

    IHC staining of human breast cancer using 66389-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FKBPL Monoclonal antibody (66389-1-Ig)

    IHC staining of human breast cancer using 66389-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using FKBPL Monoclonal antibody (66389-1-Ig)

    IHC staining of human testis using 66389-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using FKBPL Monoclonal antibody (66389-1-Ig)

    IHC staining of human testis using 66389-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66389-1-Ig (FKBPL Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FKBPL Monoclonal antibody (66389-1-Ig)

    IF Staining of MCF-7 using 66389-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66389-1-Ig (FKBPL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using FKBPL Monoclonal antibody (66389-1-Ig)

    FC experiment of HeLa using 66389-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Monoclonal antibody (66389-1-Ig, Clone:2H10B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inMCF-10A cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, Calu-3 cells, Caco-2 cells, HepG2 cells, C6 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66389-1-Ig targets FKBPL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBPL fusion protein Ag0112 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein like
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC004168
Gene symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2881765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

Protocols

Product Specific Protocols
WB protocol for FKBPL antibody 66389-1-IgDownload protocol
IHC protocol for FKBPL antibody 66389-1-IgDownload protocol
IF protocol for FKBPL antibody 66389-1-IgDownload protocol
FC protocol for FKBPL antibody 66389-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

A placenta-on-a-chip model to determine the regulation of FKBPL and galectin-3 in preeclampsia

Authors - Sahar Masoumeh Ghorbanpour
View Article