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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FLAD1 Polyclonal antibody

FLAD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14118-1-AP

Synonyms

FADS, FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using FLAD1 Polyclonal antibody (14118-1-AP)

    WB analysis of K-562 using 14118-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14118-1-AP (FLAD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FLAD1 Polyclonal antibody (14118-1-AP)

    WB analysis using 14118-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14118-1-AP (FLAD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FLAD1 Polyclonal antibody (14118-1-AP)

    WB analysis of HepG2 using 14118-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14118-1-AP (FADS antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using FLAD1 Polyclonal antibody (14118-1-AP)

    WB analysis of rat liver using 14118-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 14118-1-AP (FADS antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using FLAD1 Polyclonal antibody (14118-1-AP)

    IP experiment of K-562 using 14118-1-AP

    IP result of anti-FLAD1 (IP:14118-1-AP, 4ug; Detection:14118-1-AP 1:1000) with K-562 cells lysate 2400 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FLAD1 Polyclonal antibody (14118-1-AP)

    IF Staining of HepG2 using 14118-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FLAD1 antibody (14118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, rat liver tissue, L02 cells, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14118-1-AP targets FLAD1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLAD1 fusion protein Ag5267 相同性解析による交差性が予測される生物種
Full Name FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
Calculated molecular weight 446 aa, 49 kDa
Observed molecular weight 50 kDa, 65-70 kDa
GenBank accession numberBC011378
Gene symbol FLAD1
Gene ID (NCBI) 80308
RRIDAB_2878017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).

Protocols

Product Specific Protocols
WB protocol for FLAD1 antibody 14118-1-APDownload protocol
IF protocol for FLAD1 antibody 14118-1-APDownload protocol
IP protocol for FLAD1 antibody 14118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article