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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AAGAB Polyclonal antibody

AAGAB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27319-1-AP

Synonyms

AAGAB, FLJ11506

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using AAGAB Polyclonal antibody (27319-1-AP)

    WB analysis of K-562 using 27319-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27319-1-AP (AAGAB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using AAGAB Polyclonal antibody (27319-1-AP)

    WB analysis of mouse thymus using 27319-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 27319-1-AP (AAGAB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using AAGAB Polyclonal antibody (27319-1-AP)

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using AAGAB Polyclonal antibody (27319-1-AP)

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using AAGAB Polyclonal antibody (27319-1-AP)

    IHC staining of rat thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using AAGAB Polyclonal antibody (27319-1-AP)

    IHC staining of rat thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, mouse thymus tissue
Positive IHC detected inmouse thymus tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27319-1-AP is AAGAB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AAGAB fusion protein Ag26340 相同性解析による交差性が予測される生物種
Full Name alpha- and gamma-adaptin-binding protein p34
Observed molecular weight 38 kDa
GenBank accession numberBC058886
Gene symbol AAGAB
Gene ID (NCBI) 79719
RRIDAB_2880841
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Recent study indicate that mutations in AAGAB can cause punctate palmoplantar keratoderma type I (PPKP1; OMIM#148600), also called keratosis punctata palmoplantaris Buschke-Fischer-Brauer. The predicted MW of this protein is 35 kDa. Catalog#27319-1-AP recognises 37-40 kDa band may due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for AAGAB antibody 27319-1-APDownload protocol
IHC protocol for AAGAB antibody 27319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols