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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FLNA Monoclonal antibody (67133-1-Ig)

    WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FLNA Monoclonal antibody (67133-1-Ig)

    WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using FLNA Monoclonal antibody (67133-1-Ig)

    WB analysis of MCF-7 using 67133-1-Ig (same clone as 67133-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using FLNA Monoclonal antibody (67133-1-Ig)

    WB analysis of human skeletal muscle using 67133-1-Ig (same clone as 67133-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FLNA Monoclonal antibody (67133-1-Ig)

    WB analysis of HEK-293 using 67133-1-Ig (same clone as 67133-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FLNA Monoclonal antibody (67133-1-Ig)

    IHC staining of human cervical cancer using 67133-1-Ig (same clone as 67133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FLNA Monoclonal antibody (67133-1-Ig)

    IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FLNA Monoclonal antibody (67133-1-Ig)

    IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FLNA Monoclonal antibody (67133-1-Ig)

    IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FLNA Monoclonal antibody (67133-1-Ig)

    IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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FLNA Monoclonal antibody, PBS Only

FLNA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67133-1-PBS
Clone No.1G4H3

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67133-1-PBS targets FLNA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FLNA fusion protein Ag23291 相同性解析による交差性が予測される生物種
Full Name filamin A, alpha (actin binding protein 280)
Calculated molecular weight 2647 aa, 280 kDa
Observed molecular weight 280 kDa
GenBank accession numberBC014654
Gene Symbol FLNA
Gene ID (NCBI) 2316
RRIDAB_2882432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21333
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)