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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Filamin B Polyclonal antibody

Filamin B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20685-1-AP

Synonyms

FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FLNB Polyclonal antibody (20685-1-AP)

    WB analysis of HeLa using 20685-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FLNB Polyclonal antibody (20685-1-AP)

    WB analysis of NIH/3T3 using 20685-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using FLNB Polyclonal antibody (20685-1-AP)

    WB analysis of U2OS using 20685-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 20685-1-AP (FLNB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FLNB Polyclonal antibody (20685-1-AP)

    IHC staining of human liver cancer using 20685-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FLNB Polyclonal antibody (20685-1-AP)

    IHC staining of human liver cancer using 20685-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Filamin B Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using FLNB Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using FLNB Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using FLNB Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using FLNB Polyclonal antibody (20685-1-AP)

    IHC staining of human colon using 20685-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20685-1-AP (FLNB antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FLNB Polyclonal antibody (20685-1-AP)

    IF Staining of HepG2 using 20685-1-AP

    Immunofluorescent analysis of HepG2 cells, using FLNB antibody 20685-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, U2OS cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human normal colon, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20685-1-AP targets Filamin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name filamin B, beta (actin binding protein 278)
Calculated molecular weight 278 kDa
Observed molecular weight 250-278 kDa
GenBank accession numberNM_001457
Gene symbol FLNB
Gene ID (NCBI) 2317
RRIDAB_10863658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.

Protocols

Product Specific Protocols
WB protocol for Filamin B antibody 20685-1-APDownload protocol
IHC protocol for Filamin B antibody 20685-1-APDownload protocol
IF protocol for Filamin B antibody 20685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.

Authors - Wenwen Chen
View Article
mouseIHC

J Genet Genomics

Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts

Authors - Wenbin Huang
View Article
human,mouseIHC,IF

Am J Cancer Res

FLNB overexpression promotes tumor progression and associates with immune suppression, evasion and stemness in pancreatic cancer

Authors - Guoyong Fan
View Article