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- KD/KO Validated
Flotillin 1 Polyclonal antibody
Flotillin 1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15571-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 48 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 3 publications below |
| CoIP | See 2 publications below |
Product Information
15571-1-AP targets Flotillin 1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Flotillin 1 fusion protein Ag7902 相同性解析による交差性が予測される生物種 |
| Full Name | flotillin 1 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC001146 |
| Gene symbol | Flotillin 1 |
| Gene ID (NCBI) | 10211 |
| RRID | AB_2106746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IHC protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IP protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Theranostics Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization | ||
Cell Res Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death. | ||
Int J Biol Sci FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling
| ||
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Guorong (Verified Customer) (03-22-2022) | Good performance with a specific band of expected size and 2 bigger and weaker bands
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