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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FLT3 Polyclonal antibody (30297-1-AP)

    WB analysis using 30297-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30297-1-AP (FLT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of THP-1 cells using FLT3 Polyclonal antibody (30297-1-AP)

    IP experiment of THP-1 using 30297-1-AP

    IP result of anti-FLT3 (IP:30297-1-AP, 4ug; Detection:30297-1-AP 1:500) with THP-1 cells lysate 2720 ug.

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FLT3 Polyclonal antibody

FLT3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30297-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

FLK-2, FLK2, FL cytokine receptor, Fetal liver kinase-2, EC:2.7.10.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells
Positive IP detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30297-1-AP targets FLT3 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLT3 fusion protein Ag32898 相同性解析による交差性が予測される生物種
Full Name fms-related tyrosine kinase 3
Calculated molecular weight112 kDa
Observed molecular weight130 kDa, 150 kDa
GenBank accession numberBC126350
Gene Symbol FLT3
Gene ID (NCBI) 2322
RRIDAB_3086289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Protocols

Product Specific Protocols
WB protocol for FLT3 antibody 30297-1-APDownload protocol
IP protocol for FLT3 antibody 30297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols