Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of Jurkat using 26841-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 26841-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 26841-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 26841-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 26841-1-AP
IP result of anti-FLVCR1 (IP:26841-1-AP, 4ug; Detection:26841-1-AP 1:500) with K-562 cells lysate 4400 ug.
IHC staining of human breast cancer using 26841-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 26841-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Caco-2 using 26841-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using 26841-1-AP (FLVCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

FLVCR1 Polyclonal antibody

FLVCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 26841-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Heme transporter FLVCR1, FLVCR, Feline leukemia virus subgroup C receptor-related protein 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HepG2 cells, human placenta tissue, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human breast cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Caco-2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

26841-1-AP targets FLVCR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FLVCR1 fusion protein Ag25220 相同性解析による交差性が予測される生物種
Full Name	feline leukemia virus subgroup C cellular receptor 1
Calculated molecular weight	60 kDa
Observed molecular weight	60 kDa~70 kDa
GenBank accession number	BC048312
Gene Symbol	FLVCR1
Gene ID (NCBI)	28982
RRID	AB_2880654
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y5Y0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FLVCR1(feline leukemia virus subgroup C receptor 1) has been reported to have a crucial role in a variety of biological processes, including cell proliferation, cell death, apoptosis, oxidative stress response, cellular differentiation, and metabolism (PMID: 29532854). In addition, FLVCR1 can be applied as a clinical prognostic marker for patients with ESCC (PMID: 33842377).

Protocols

Product Specific Protocols
WB protocol for FLVCR1 antibody 26841-1-AP	Download protocol
IHC protocol for FLVCR1 antibody 26841-1-AP	Download protocol
IF protocol for FLVCR1 antibody 26841-1-AP	Download protocol
IP protocol for FLVCR1 antibody 26841-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Nanobiotechnology Apoptotic metabolites ameliorate bone aging phenotypes via TCOF1/FLVCR1-mediated mitochondrial homeostasis Authors - Yan Qu KD Validated View Article
		Cell Rep Flvcr1a deficiency promotes heme-based energy metabolism dysfunction in skeletal muscle Authors - Miriam Mistretta View Article
human,mouse	WB,IP,IF	Cell Rep Med Dysregulation of FLVCR1a-dependent mitochondrial calcium handling in neural progenitors causes congenital hydrocephalus Authors - Francesca Bertino KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 26841-1-AP

WB analysis of human placenta using 26841-1-AP

WB analysis of K-562 using 26841-1-AP

WB analysis of HepG2 using 26841-1-AP

IP experiment of K-562 using 26841-1-AP

IHC staining of human breast cancer using 26841-1-AP

IHC staining of mouse kidney using 26841-1-AP

IF Staining of Caco-2 using 26841-1-AP