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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FLVCR1 Polyclonal antibody

FLVCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26841-1-AP

Synonyms

Heme transporter FLVCR1, FLVCR, Feline leukemia virus subgroup C receptor-related protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using FLVCR1 Polyclonal antibody (26841-1-AP)

    WB analysis of Jurkat using 26841-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FLVCR1 Polyclonal antibody (26841-1-AP)

    WB analysis of human placenta using 26841-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FLVCR1 Polyclonal antibody (26841-1-AP)

    WB analysis of K-562 using 26841-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FLVCR1 Polyclonal antibody (26841-1-AP)

    WB analysis of HepG2 using 26841-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26841-1-AP (FLVCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using FLVCR1 Polyclonal antibody (26841-1-AP)

    IP experiment of K-562 using 26841-1-AP

    IP result of anti-FLVCR1 (IP:26841-1-AP, 4ug; Detection:26841-1-AP 1:500) with K-562 cells lysate 4400 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FLVCR1 Polyclonal antibody (26841-1-AP)

    IHC staining of human breast cancer using 26841-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FLVCR1 Polyclonal antibody (26841-1-AP)

    IHC staining of mouse kidney using 26841-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26841-1-AP (FLVCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using FLVCR1 Polyclonal antibody (26841-1-AP)

    IF Staining of Caco-2 using 26841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using 26841-1-AP (FLVCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, human placenta tissue, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26841-1-AP targets FLVCR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLVCR1 fusion protein Ag25220 相同性解析による交差性が予測される生物種
Full Name feline leukemia virus subgroup C cellular receptor 1
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa~70 kDa
GenBank accession numberBC048312
Gene symbol FLVCR1
Gene ID (NCBI) 28982
RRIDAB_2880654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLVCR1(feline leukemia virus subgroup C receptor 1) has been reported to have a crucial role in a variety of biological processes, including cell proliferation, cell death, apoptosis, oxidative stress response, cellular differentiation, and metabolism (PMID: 29532854). In addition, FLVCR1 can be applied as a clinical prognostic marker for patients with ESCC (PMID: 33842377).

Protocols

Product Specific Protocols
WB protocol for FLVCR1 antibody 26841-1-APDownload protocol
IHC protocol for FLVCR1 antibody 26841-1-APDownload protocol
IF protocol for FLVCR1 antibody 26841-1-APDownload protocol
IP protocol for FLVCR1 antibody 26841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Rep

Flvcr1a deficiency promotes heme-based energy metabolism dysfunction in skeletal muscle

Authors - Miriam Mistretta
View Article
human,mouseWB,IP,IF

Cell Rep Med

Dysregulation of FLVCR1a-dependent mitochondrial calcium handling in neural progenitors causes congenital hydrocephalus

Authors - Francesca Bertino
  • KD Validated
View Article