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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using FMN1 Polyclonal antibody (25982-1-AP)

    WB analysis of A549 using 25982-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25982-1-AP (FMN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FMN1 Polyclonal antibody (25982-1-AP)

    IF Staining of A549 using 25982-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FMN1 antibody (25982-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FMN1 Polyclonal antibody

FMN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25982-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Limb deformity protein homolog, LD, Formin-1, FMN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25982-1-AP targets FMN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMN1 fusion protein Ag23059 相同性解析による交差性が予測される生物種
Full Name formin 1
Calculated molecular weight 1196 aa, 132 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC103692
Gene Symbol FMN1
Gene ID (NCBI) 342184
RRIDAB_2880319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68DA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FMN1 antibody 25982-1-APDownload protocol
IF protocol for FMN1 antibody 25982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Molecules

MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway

Authors - Pan Xu
View Article