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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FMNL1 Monoclonal antibody, PBS Only

FMNL1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12A4

Cat no : 67587-1-PBS

Synonyms

Formin-like protein 1, FMNL, CLL-associated antigen KW-13, 1B12A4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis using 67587-1-Ig (same clone as 67587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis using 67587-1-Ig (same clone as 67587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis of Jurkat using 67587-1-Ig (same clone as 67587-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NK-92 cells using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis of NK-92 using 67587-1-Ig (same clone as 67587-1-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FMNL1 Monoclonal antibody (67587-1-Ig)

    IF Staining of U2OS using 67587-1-Ig (same clone as 67587-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (67587-1-Ig, Clone: 1B12A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67587-1-PBS targets FMNL1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FMNL1 fusion protein Ag27322 相同性解析による交差性が予測される生物種
Full Name formin-like 1
Observed molecular weight 140-150 kDa
GenBank accession numberBC111564
Gene symbol FMNL1
Gene ID (NCBI) 752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 67587-1-Ig antibody detects the protein around 140-150 kDa which is similar to the results reported in publications.(PMID: 21868368, 22986496 , 23182705)