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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FMNL2 Monoclonal antibody

FMNL2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E8C12

Cat no : 68551-1-Ig

Synonyms

FHOD2, FLJ37546, FMNL2, formin like 2, Formin like protein 2, KIAA1902

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMNL2 Monoclonal antibody (68551-1-Ig)

    WB analysis using 68551-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68551-1-Ig (FMNL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of hTERT-RPE1 cells using FMNL2 Monoclonal antibody (68551-1-Ig)

    WB analysis of hTERT-RPE1 using 68551-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68551-1-Ig (FMNL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, hTERT-RPE1 cells, HEK-293 cells, A549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68551-1-Ig targets FMNL2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FMNL2 fusion protein Ag34164 相同性解析による交差性が予測される生物種
Full Name formin-like 2
Observed molecular weight140-150 kDa
GenBank accession numberBC167159
Gene symbol FMNL2
Gene ID (NCBI) 114793
RRIDAB_3085255
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Formin-like 2 (FMNL2), also known as FRL3 or FHOD2, a member of the diaphanous-related formins, contains a GTPase-binding domain and autoregulatory domains, and is proposed to function as a downstream effector of Rho family guanosine triphosphatases (GTPases) (PMID:15950879). FMNL2 mRNA is expressed in many normal tissues and dysregulated in several cancers such as colorectal cancer, melanoma and involved in invasive behaviours and progression of cancer cells.

Protocols

Product Specific Protocols
WB protocol for FMNL2 antibody 68551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols