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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMNL3 Polyclonal antibody (29756-1-AP)

    WB analysis using 29756-1-AP (same clone as 29756-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29756-1-AP (FMNL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of human colon cancer using 29756-1-AP (same clone as 29756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of human colon cancer using 29756-1-AP (same clone as 29756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of mouse liver using 29756-1-AP (same clone as 29756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of mouse liver using 29756-1-AP (same clone as 29756-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using FMNL3 Polyclonal antibody (29756-1-AP)

    IF Staining of HUVEC using 29756-1-AP (same clone as 29756-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FMNL3 antibody (29756-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

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FMNL3 Polyclonal antibody, PBS Only

FMNL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29756-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29756-1-PBS targets FMNL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMNL3 fusion protein Ag30543 相同性解析による交差性が予測される生物種
Full Name formin-like 3
Calculated molecular weight 117 kDa
Observed molecular weight117 kDa
GenBank accession numberNM_175736
Gene Symbol FMNL3
Gene ID (NCBI) 91010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVF7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FMNL3 plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the control of cell shape and migration. In quiescent endothelial cells, FMNL3 triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.