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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using FMO1 Polyclonal antibody (30977-1-AP)

    IHC staining of human liver using 30977-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30977-1-AP (FMO1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using FMO1 Polyclonal antibody (30977-1-AP)

    IHC staining of human liver using 30977-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30977-1-AP (FMO1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FMO1 Polyclonal antibody (30977-1-AP)

    IHC staining of mouse kidney using 30977-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30977-1-AP (FMO1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FMO1 Polyclonal antibody (30977-1-AP)

    IHC staining of mouse kidney using 30977-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30977-1-AP (FMO1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FMO1 Polyclonal antibody

FMO1 Polyclonal Antibody for IHC, ELISA
Cat No. 30977-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

Trimethylamine monooxygenase, Flavin-containing monooxygenase 1, Fetal hepatic flavin-containing monooxygenase 1, EC:1.14.13.148, Dimethylaniline monooxygenase [N-oxide-forming] 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30977-1-AP targets FMO1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMO1 fusion protein Ag34267 相同性解析による交差性が予測される生物種
Full Name flavin containing monooxygenase 1
Calculated molecular weight60 kDa
GenBank accession numberNM_001282692
Gene Symbol FMO1
Gene ID (NCBI) 2326
RRIDAB_3669802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ01740
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flavin-containing monooxygenase 1 (FMO1) belongs to the FMO family. FMO1 is an enzyme that catalyzes the oxygenation of a series of sulfur-containing and nitrogen-containing substrates, and thus is involved in a series of drugs and xenobiotics metabolism. FMO1 can regulate PPAR-α and mediate metabolic processes. FMO1 is expressed at relatively high levels in human fetal liver and also represents the major FMO enzyme in the adult human and rabbit intestine and kidney (PMID: 11809920, 30757917, 37553313). Human FMO1 has 2 isoforms with the molecular weights of 53 kDa and 60 kDa.

Protocols

Product Specific Protocols
IHC protocol for FMO1 antibody 30977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols