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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FMO2 Polyclonal antibody

FMO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15725-1-AP

Synonyms

Dimethylaniline oxidase 2, FMO 1B1, FMO 2, FMO1B1, FMO2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMO2 Polyclonal antibody (15725-1-AP)

    WB analysis using 15725-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15725-1-AP (FMO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FMO2 Polyclonal antibody (15725-1-AP)

    IHC staining of human lung cancer using 15725-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FMO2 Polyclonal antibody (15725-1-AP)

    IHC staining of human lung cancer using 15725-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15725-1-AP targets FMO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMO2 fusion protein Ag8464 相同性解析による交差性が予測される生物種
Full Name flavin containing monooxygenase 2
Calculated molecular weight471aa,54 kDa; 534aa,61 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC005894
Gene symbol FMO2
Gene ID (NCBI) 2327
RRIDAB_2278518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FMO2, belongs to FMO (flavin containing monooxygenase) family, which catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. FMO family has 1-5 members, with homologous sequences. This antibody is specially against FMO2. It does not bind other FMOs.

Protocols

Product Specific Protocols
WB protocol for FMO2 antibody 15725-1-APDownload protocol
IHC protocol for FMO2 antibody 15725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols