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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FMO2 Monoclonal antibody

FMO2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3F1F7

Cat no : 67019-1-Ig

Synonyms

Dimethylaniline oxidase 2, FLJ40826, FMO 1B1, FMO 2, FMO1B1, FMO2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using FMO2 Monoclonal antibody (67019-1-Ig)

    WB analysis of mouse lung using 67019-1-Ig

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 67019-1-Ig (FMO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using FMO2 Monoclonal antibody (67019-1-Ig)

    WB analysis of A549 using 67019-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67019-1-Ig (FMO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig lung tissue using FMO2 Monoclonal antibody (67019-1-Ig)

    WB analysis of pig lung using 67019-1-Ig

    pig lung tissue were subjected to SDS PAGE followed by western blot with 67019-1-Ig (FMO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FMO2 Monoclonal antibody (67019-1-Ig)

    IHC staining of human lung cancer using 67019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67019-1-Ig (FMO2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FMO2 Monoclonal antibody (67019-1-Ig)

    IHC staining of human lung cancer using 67019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67019-1-Ig (FMO2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FMO2 Monoclonal antibody (67019-1-Ig)

    IF Staining of A549 using 67019-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FMO2 antibody (67019-1-Ig, Clone: 3F1F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inmouse lung tissue, A549 cells, pig lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67019-1-Ig targets FMO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity Human, Mouse, Pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FMO2 fusion protein Ag8610 相同性解析による交差性が予測される生物種
Full Name flavin containing monooxygenase 2
Calculated molecular weight471aa,54 kDa; 534aa,61 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC005894
Gene symbol FMO2
Gene ID (NCBI) 2327
RRIDAB_2882335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FMO2 antibody 67019-1-IgDownload protocol
IHC protocol for FMO2 antibody 67019-1-IgDownload protocol
IF protocol for FMO2 antibody 67019-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism.

Authors - Shijiao Huang
View Article
humanIF

Cells

Differentiation Trajectory of Limbal Stem and Progenitor Cells under Normal Homeostasis and upon Corneal Wounding.

Authors - Zhenwei Song
View Article