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  • Featured Product
  • KD/KO Validated

FMO5-specific Polyclonal antibody

FMO5-specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 16864-1-AP

Synonyms

FMO5, Flavin-containing monooxygenase 5, EC:1.6.3.1, EC:1.14.13.8, EC:1.14.13.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMO5-specific Polyclonal antibody (16864-1-AP)

    WB analysis using 16864-1-AP

    PC-3 cells and LNCap cells were subjected to SDS PAGE followed by western blot with 16864-1-AP (FMO5-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FMO5-specific Polyclonal antibody (16864-1-AP)

    WB analysis using 16864-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16864-1-AP (FMO5-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of multi-cells using FMO5-specific Polyclonal antibody (16864-1-AP)

    WB analysis of multi-cells using 16864-1-AP

    WB result of 16864-1-AP from Dr.Soory.

  • Immunohistochemistry (IHC) staining of human liver tissue using FMO5-specific Polyclonal antibody (16864-1-AP)

    IHC staining of human liver using 16864-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using FMO5-specific Polyclonal antibody (16864-1-AP)

    IHC staining of human liver using 16864-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FMO5-specific Polyclonal antibody (16864-1-AP)

    IHC staining of mouse liver using 16864-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FMO5-specific Polyclonal antibody (16864-1-AP)

    IHC staining of mouse liver using 16864-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16864-1-AP (FMO5-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FMO5-specific Polyclonal antibody (16864-1-AP)

    IHC staining of human breast cancer using 16864-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FMO5-specific Polyclonal antibody (16864-1-AP)

    IHC staining of human breast cancer using 16864-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FMO5-specific Polyclonal antibody (16864-1-AP)

    IF Staining of MCF-7 using 16864-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 16864-1-AP (FMO5-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using FMO5-specific Polyclonal antibody (16864-1-AP)

    FC experiment of MCF-7 using 16864-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug FMO5-specific Polyclonal antibody (16864-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inPC-3 cells, mouse liver tissue, DU 145 cells, LNCaP cells, VCaP cells, RAW 264.7 cells, MCF-7 cells, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16864-1-AP targets FMO5-specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name flavin containing monooxygenase 5
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_001461
Gene symbol FMO5
Gene ID (NCBI) 2330
RRIDAB_11125955
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FMO5-specific antibody 16864-1-APDownload protocol
IHC protocol for FMO5-specific antibody 16864-1-APDownload protocol
IF protocol for FMO5-specific antibody 16864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Drug Metab Dispos

Identification of flavin-containing monooxygenase 5 (FMO5) as a regulator of glucose homeostasis and a potential sensor of gut bacteria.

Authors - Flora Scott
  • KO Validated
View Article
mouseIF

J Transl Med

Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism

Authors - Yi-Xi Li
View Article