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FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13755-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

POF, FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inK-562 cells, mouse brain tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human liver tissue, human colon cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13755-1-AP targets FMR1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMR1 fusion protein Ag4697 相同性解析による交差性が予測される生物種
Full Name fragile X mental retardation 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight 59-72 kDa
GenBank accession numberBC038998
Gene Symbol FMR1
Gene ID (NCBI) 2332
RRIDAB_2262872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Protocols

Product Specific Protocols
WB protocol for FMR1 antibody 13755-1-APDownload protocol
IHC protocol for FMR1 antibody 13755-1-APDownload protocol
IF protocol for FMR1 antibody 13755-1-APDownload protocol
IP protocol for FMR1 antibody 13755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
humanWB

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang
humanWB

Int Immunopharmacol

MNSFβ promotes LPS-induced TNFα expression by increasing the localization of RC3H1 to stress granules, and the interfering peptide HEPN2 reduces TNFα production by disrupting the MNSFβ-RC3H1 interaction in macrophages

Authors - Han-Yu Jiang
humanWB

Cancer Cell Int

MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.

Authors - Yu Men
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
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