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FMR1 Monoclonal antibody, PBS Only

Name: FMR1 Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 66548-1-PBS

FMR1 Monoclonal Antibody for Indirect ELISA, IP, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

rat, mouse, human

Applications

Indirect ELISA, IP, IHC, WB

Conjugate

Unconjugated

CloneNo.

2B5B5

Review Product

Cat no : 66548-1-PBS

Synonyms

FRAXA, Fragile X messenger ribonucleoprotein 1, Fragile X messenger ribonucleoprotein, fragile X mental retardation 1, FMRP

Validation Data Gallery

Filter:

AllWBIPIHCFC (Intra)

WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66548-1-Ig (FMR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)
IP result of anti-FMR1 (IP:66548-1-Ig, 5ug; Detection:66548-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
×
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
×
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

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Cat No. 66548-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66548-1-PBS targets FMR1 in Indirect ELISA, IP, IHC, WB applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity	rat, mouse, human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	FMR1 fusion protein Ag4697 相同性解析による交差性が予測される生物種
Full Name	fragile X mental retardation 1
Calculated molecular weight	632 aa, 71 kDa
Observed molecular weight	69-72 kDa
GenBank accession number	BC038998
Gene symbol	FMR1
Gene ID (NCBI)	2332
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FMR1 Monoclonal antibody, PBS Only

FMR1 Monoclonal Antibody for Indirect ELISA, IP, IHC, WB

Validation Data Gallery

WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)

IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)

IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)