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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FMR1NB Polyclonal antibody

FMR1NB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11069-2-AP

Synonyms

Cancer/testis antigen 37, CT37, FMR1NB, NY SAR 35, Sarcoma antigen NY SAR 35

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using FMR1NB Polyclonal antibody (11069-2-AP)

    WB analysis of MCF-7 using 11069-2-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using FMR1NB Polyclonal antibody (11069-2-AP)

    WB analysis of SKOV-3 using 11069-2-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FMR1NB Polyclonal antibody (11069-2-AP)

    WB analysis of MCF-7 using 11069-2-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using FMR1NB Polyclonal antibody (11069-2-AP)

    WB analysis of SKOV-3 using 11069-2-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using FMR1NB Polyclonal antibody (11069-2-AP)

    WB analysis of A549 using 11069-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FMR1NB Polyclonal antibody (11069-2-AP)

    IHC staining of mouse testis using 11069-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11069-2-AP (FMR1NB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMCF7 cells, A549 cells, MCF-7 cells, SKOV-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11069-2-AP targets FMR1NB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMR1NB fusion protein Ag1531 相同性解析による交差性が予測される生物種
Full Name fragile X mental retardation 1 neighbor
Calculated molecular weight 29 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034320
Gene symbol FMR1NB
Gene ID (NCBI) 158521
RRIDAB_2105567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FMR1NB antibody 11069-2-APDownload protocol
IHC protocol for FMR1NB antibody 11069-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols