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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FN3K Polyclonal antibody

FN3K Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14293-1-AP

Synonyms

Protein-ribulosamine 3-kinase FN3K, Protein-psicosamine 3-kinase FN3K, Fructosamine-3-kinase, fructosamine 3 kinase, EC:2.7.1.172

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FN3K Polyclonal antibody (14293-1-AP)

    WB analysis of HeLa using 14293-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using FN3K Polyclonal antibody (14293-1-AP)

    WB analysis of human heart using 14293-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FN3K Polyclonal antibody (14293-1-AP)

    WB analysis of human brain using 14293-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using FN3K Polyclonal antibody (14293-1-AP)

    WB analysis of human kidney using 14293-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using FN3K Polyclonal antibody (14293-1-AP)

    WB analysis of mouse skeletal muscle using 14293-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FN3K Polyclonal antibody (14293-1-AP)

    IP experiment of HeLa using 14293-1-AP

    IP result of anti-FN3K (IP:14293-1-AP, 3ug; Detection:14293-1-AP 1:500) with HeLa cells lysate 1840ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FN3K Polyclonal antibody (14293-1-AP)

    IHC staining of human kidney using 14293-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FN3K Polyclonal antibody (14293-1-AP)

    IHC staining of human kidney using 14293-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FN3K Polyclonal antibody (14293-1-AP)

    IF Staining of HeLa using 14293-1-AP

    Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, human kidney tissue, mouse skeletal muscle tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14293-1-AP targets FN3K in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FN3K fusion protein Ag5617 相同性解析による交差性が予測される生物種
Full Name fructosamine 3 kinase
Calculated molecular weight 34 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC042680
Gene symbol FN3K
Gene ID (NCBI) 64122
RRIDAB_2105975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FN3K antibody 14293-1-APDownload protocol
IHC protocol for FN3K antibody 14293-1-APDownload protocol
IF protocol for FN3K antibody 14293-1-APDownload protocol
IP protocol for FN3K antibody 14293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Amiloride reduces fructosamine-3-kinase expression to restore sunitinib sensitivity in renal cell carcinoma

Authors - Yuanyuan Bai
View Article