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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FN3KRP Polyclonal antibody (16312-1-AP)

    WB analysis using 16312-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FN3KRP Polyclonal antibody (16312-1-AP)

    WB analysis of HEK-293 using 16312-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FN3KRP Polyclonal antibody (16312-1-AP)

    WB analysis of HeLa using 16312-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FN3KRP Polyclonal antibody (16312-1-AP)

    IF Staining of HeLa using 16312-1-AP

    Immunofluorescent analysis of HeLa cells using 16312-1-AP (FN3KRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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FN3KRP Polyclonal antibody

FN3KRP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16312-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Ketosamine-3-kinase, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, EC:2.7.1.172

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16312-1-AP targets FN3KRP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FN3KRP fusion protein Ag9347 相同性解析による交差性が予測される生物種
Full Name fructosamine 3 kinase related protein
Calculated molecular weight 309 aa, 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC014408
Gene Symbol FN3KRP
Gene ID (NCBI) 79672
RRIDAB_2105986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HA64
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fn3KRP (Fn3K-related protein) are believed to be the first examples of deglycating enzymes acting on the Amadori product to reverse the early stages of the Maillard reaction in vivo in higher organisms (PMID: 17337043). Fn3KRP possesses an ATP-binding domain that is structurally related to that of both the aminoglycoside kinases and eukaryotic protein kinases. Fn3KRP possesses an intrinsic activity towards ketosamine substrates (PMID: 18637789). Catalog#16312-1-AP is a rabbit polyclonal antibody raised against the full-length of human FN3KRP. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

Protocols

Product Specific Protocols
WB protocol for FN3KRP antibody 16312-1-APDownload protocol
IF protocol for FN3KRP antibody 16312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols