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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FNDC5 Recombinant antibody (82671-1-RR)

    WB analysis using 82671-1-RR (same clone as 82671-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82671-1-RR (FNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FNDC5 Recombinant antibody (82671-1-RR)

    IHC staining of mouse skeletal muscle using 82671-1-RR (same clone as 82671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using FNDC5 Recombinant antibody (82671-1-RR)

    IHC staining of mouse skeletal muscle using 82671-1-RR (same clone as 82671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82671-1-RR (FNDC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82671-1-PBS in a different storage buffer formulation.

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FNDC5 Recombinant antibody, PBS Only

FNDC5 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82671-1-PBS
Clone No.2H3

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

FNDC5, FRCP2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82671-1-PBS targets FNDC5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FNDC5 fusion protein Ag29714 相同性解析による交差性が予測される生物種
Full Name fibronectin type III domain containing 5
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC062297
Gene Symbol FNDC5
Gene ID (NCBI) 252995
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NAU1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

fibronectin type III domain containing 5(FNDC5) encodes 23 kDa protein, which is a membrane protein that is cleaved and secreted as a newly identified hormone, irisin. Exercise-induced FNDC5 gene expression in muscles was accompanied by a parallel increase in the concentration of circulating irisin, which in turn activates adipocyte thermogenic programs, leading to mitochondrial heat production and energy expenditure.