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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FNIP1 Polyclonal antibody

FNIP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28380-1-AP

Synonyms

DKFZp686E18167, DKFZp781P0215, FNIP1, KIAA1961

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FNIP1 Polyclonal antibody (28380-1-AP)

    WB analysis using 28380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28380-1-AP (FNIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. .

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using FNIP1 Polyclonal antibody (28380-1-AP)

    IF Staining of C2C12 using 28380-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using FNIP1 antibody (28380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28380-1-AP targets FNIP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FNIP1 fusion protein Ag28976 相同性解析による交差性が予測される生物種
Full Name folliculin interacting protein 1
Calculated molecular weight 508 aa, 57 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC001956
Gene symbol FNIP1
Gene ID (NCBI) 96459
RRIDAB_2881128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Folliculin-interacting protein 1 (FNIP1) forms a complex with the protein folliculin (FLCN), together acting as guanosine triphosphate (GTP)-activating protein (GAP) for RagC. The FNIP1-FLCN complex has emerged as an amino acid sensor to the mechanistic target of rapamycin complex 1 (mTORC1), involved in how amino acids control TFEB activation. AMPK phosphorylation of FNIP1 induces lysosomal and mitochondrial biogenesis (PMID: 37079666)

Protocols

Product Specific Protocols
WB protocol for FNIP1 antibody 28380-1-APDownload protocol
IF protocol for FNIP1 antibody 28380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Bioact Mater

Glucose-responsive, antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesis

Authors - Wenqian Zhang
View Article