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  • Featured Product
  • KD/KO Validated

FNTB Polyclonal antibody

FNTB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11507-1-AP

Synonyms

FNTB, FPTB, FTase beta

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using FNTB Polyclonal antibody (11507-1-AP)

    WB analysis of mouse heart using 11507-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11507-1-AP (FNTB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using FNTB Polyclonal antibody (11507-1-AP)

    WB analysis of A431 using 11507-1-AP

    WB result of FNTB antibody (11507-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected A431 cells.

  • Western Blot (WB) analysis of mouse skin tissue using FNTB Polyclonal antibody (11507-1-AP)

    WB analysis of mouse skin using 11507-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11507-1-AP (FNTB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FNTB Polyclonal antibody (11507-1-AP)

    IP experiment of HeLa using 11507-1-AP

    IP result of anti-FNTB (IP:11507-1-AP, 4ug; Detection:11507-1-AP 1:1000) with HeLa cells lysate 800ug.

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Tested Applications

Positive WB detected inmouse heart tissue, A431 cells, mouse skin tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11507-1-AP targets FNTB in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FNTB fusion protein Ag2061 相同性解析による交差性が予測される生物種
Full Name farnesyltransferase, CAAX box, beta
Calculated molecular weight 437 aa, 49 kDa
Observed molecular weight 49 kDa, 44 kDa
GenBank accession numberBC020232
Gene symbol FNTB
Gene ID (NCBI) 2342
RRIDAB_2106301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

Protocols

Product Specific Protocols
WB protocol for FNTB antibody 11507-1-APDownload protocol
IHC protocol for FNTB antibody 11507-1-APDownload protocol
IP protocol for FNTB antibody 11507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols