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  • Featured Product
  • KD/KO Validated

FNTB Monoclonal antibody, PBS Only

FNTB Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F3B10

Cat no : 66783-1-PBS

Synonyms

FNTB, FPTB, FTase beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis of HeLa using 66783-1-Ig (same clone as 66783-1-PBS)

    WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis of HEK-293 using 66783-1-Ig (same clone as 66783-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FNTB Monoclonal antibody (66783-1-Ig)

    IF Staining of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66783-1-PBS targets FNTB in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FNTB fusion protein Ag27796 相同性解析による交差性が予測される生物種
Full Name farnesyltransferase, CAAX box, beta
Calculated molecular weight 437 aa, 49 kDa
Observed molecular weight 44-45 kDa
GenBank accession numberBC020232
Gene symbol FNTB
Gene ID (NCBI) 2342
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.