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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FOLR1 Polyclonal antibody (23355-1-AP)

    WB analysis of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

    WB result of FOLR1 antibody (23355-1-AP, 1:1000) with si-Control and si-FOLR1 transfected HeLa cells.. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FOLR1 Polyclonal antibody (23355-1-AP)

    WB analysis of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOLR1 Polyclonal antibody (23355-1-AP)

    WB analysis using 23355-1-AP (same clone as 23355-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOLR1 Polyclonal antibody (23355-1-AP)

    IHC staining of human breast cancer using 23355-1-AP (same clone as 23355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOLR1 Polyclonal antibody (23355-1-AP)

    IHC staining of human breast cancer using 23355-1-AP (same clone as 23355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FOLR1 Polyclonal antibody (23355-1-AP)

    IF Staining of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOLR1 antibody (23355-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FOLR1 Polyclonal antibody, PBS Only

FOLR1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23355-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23355-1-PBS targets FOLR1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOLR1 fusion protein Ag19959 相同性解析による交差性が予測される生物種
Full Name folate receptor 1 (adult)
Calculated molecular weight 257 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC002947
Gene Symbol FOLR1
Gene ID (NCBI) 2348
RRIDAB_2879264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15328
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).