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  • Featured Product
  • KD/KO Validated

FOLR1 Polyclonal antibody, PBS Only

FOLR1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23355-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FOLR1 Polyclonal antibody (23355-1-AP)

    WB analysis of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

    WB result of FOLR1 antibody (23355-1-AP, 1:1000) with si-Control and si-FOLR1 transfected HeLa cells.. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOLR1 Polyclonal antibody (23355-1-AP)

    WB analysis using 23355-1-AP (same clone as 23355-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FOLR1 Polyclonal antibody (23355-1-AP)

    WB analysis of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23355-1-AP (FOLR1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOLR1 Polyclonal antibody (23355-1-AP)

    IHC staining of human breast cancer using 23355-1-AP (same clone as 23355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOLR1 Polyclonal antibody (23355-1-AP)

    IHC staining of human breast cancer using 23355-1-AP (same clone as 23355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23355-1-AP (FOLR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FOLR1 Polyclonal antibody (23355-1-AP)

    IF Staining of HeLa using 23355-1-AP (same clone as 23355-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOLR1 antibody (23355-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23355-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23355-1-PBS targets FOLR1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOLR1 fusion protein Ag19959 相同性解析による交差性が予測される生物種
Full Name folate receptor 1 (adult)
Calculated molecular weight 257 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC002947
Gene symbol FOLR1
Gene ID (NCBI) 2348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).