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c-Fos Polyclonal antibody

c-Fos Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26192-1-AP

Synonyms

AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos



Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26192-1-AP is c-Fos Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-Fos fusion protein Ag24340 相同性解析による交差性が予測される生物種
Full Name FOS
Calculated molecular weight 41 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC004490
Gene symbol FOS
Gene ID (NCBI) 2353
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

Protocols

Product Specific Protocols
WB protocol for c-Fos antibody 26192-1-APDownload protocol
IHC protocol for c-Fos antibody 26192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC, IF

Nature

Mechanisms of stretch-mediated skin expansion at single-cell resolution.

Authors - Mariaceleste Aragona
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouseIHC

Nat Commun

Ketamine activates adult-born immature granule neurons to rapidly alleviate depression-like behaviors in mice.

Authors - Radhika Rawat
mouseIHC

Biol Psychiatry

Fighting Females: Neural and Behavioral Consequences of Social Defeat Stress in Female Mice.

Authors - Emily L Newman
humanIHC

Nat Commun

Excitatory VTA to DH projections provide a valence signal to memory circuits.

Authors - Yuan Han
mouseIHC

Nat Commun

Activating newborn neurons suppresses depression and anxiety-like behaviors.

Authors - Elif Tunc-Ozcan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Matthias (Verified Customer) (09-11-2019)

Good Antibody, very specific.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 2000
  • Cell Tissue Type: Brain tissue
c-Fos Antibody Immunohistochemistry,Immunofluorescence, validation (2000 dilution) in Brain tissue (Cat no:26192-1-AP)
FH

Elif (Verified Customer) (06-21-2019)

It works better with 2 overnight incubation in 4 degrees.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Dentate gyrus
c-Fos Antibody Immunohistochemistry, validation (1:500 dilution) in Dentate gyrus (Cat no:26192-1-AP)