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  • Featured Product
  • KD/KO Validated

FOX2/RBM9 Monoclonal antibody, PBS Only

FOX2/RBM9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7G5

Cat no : 66976-1-PBS

Synonyms

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, FOX2/RBM9, fxh, HNRBP2, HRNBP2, RBFOX2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis using 66976-1-Ig (same clone as 66976-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of HEK-293 using 66976-1-Ig (same clone as 66976-1-PBS)

    WB result of RBM9 antibody (66976-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of HT-29 using 66976-1-Ig (same clone as 66976-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of NCCIT using 66976-1-Ig (same clone as 66976-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of fetal human brain using 66976-1-Ig (same clone as 66976-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of human placenta using 66976-1-Ig (same clone as 66976-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human lung cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human lung cancer using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human ovary tumor using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human ovary tumor using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66976-1-PBS targets FOX2/RBM9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOX2/RBM9 fusion protein Ag28336 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 9
Calculated molecular weight 390 aa, 41 kDa
Observed molecular weight 48 kDa, 50 and 60 kDa
GenBank accession numberBC013115
Gene symbol RBM9
Gene ID (NCBI) 23543
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)