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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXC1 Polyclonal antibody

FOXC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30082-1-AP

Synonyms

FREAC3, FREAC 3, Forkhead-related transcription factor 3, Forkhead-related protein FKHL7, FKHL7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXC1 Polyclonal antibody (30082-1-AP)

    WB analysis using 30082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30082-1-AP (FOXC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30082-1-AP targets FOXC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXC1 fusion protein Ag32531 相同性解析による交差性が予測される生物種
Full Name forkhead box C1
Calculated molecular weight57kd
Observed molecular weight 70 kDa
GenBank accession numberNM_001453.3
Gene symbol FOXC1
Gene ID (NCBI) 2296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly. This antibody is specific to FOXC1. Phosphorylation modification of Foxc1 protein may be responsible for the larger molecular weight of detection compared with theoretical molecular weight (PMID:27708239;16403239).

Protocols

Product Specific Protocols
WB protocol for FOXC1 antibody 30082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols