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  • Featured Product
  • KD/KO Validated

FOXC1 Polyclonal antibody, PBS Only

FOXC1 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55365-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FOXC1 Polyclonal antibody (55365-1-AP)

    WB analysis of HEK-293 using 55365-1-AP (same clone as 55365-1-PBS)

    WB result of FOXC1 antibody (55365-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using FOXC1 Polyclonal antibody (55365-1-AP)

    WB analysis of MCF-7 using 55365-1-AP (same clone as 55365-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FOXC1 Polyclonal antibody (55365-1-AP)

    WB analysis of HEK-293 using 55365-1-AP (same clone as 55365-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FOXC1 Polyclonal antibody (55365-1-AP)

    WB analysis of HEK-293 using 55365-1-AP (same clone as 55365-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FOXC1 Polyclonal antibody (55365-1-AP)

    WB analysis of HepG2 using 55365-1-AP (same clone as 55365-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55365-1-AP (FOXC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using FOXC1 Polyclonal antibody (55365-1-AP)

    IP experiment of HepG2 using 55365-1-AP (same clone as 55365-1-PBS)

    IP result of anti-FOXC1 (IP:55365-1-AP, 4ug; Detection:55365-1-AP 1:300) with HepG2 cells lysate 4000 ug. This data was developed using the same antibody clone with 55365-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55365-1-PBS targets FOXC1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name forkhead box C1
Calculated molecular weight 57 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_001453
Gene symbol FOXC1
Gene ID (NCBI) 2296
RRIDAB_2881316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly. This antibody is specific to FOXC1. Phosphorylation modification of Foxc1 protein may be responsible for the larger molecular weight of detection compared with theoretical molecular weight (PMID:27708239;16403239).