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Validation Data Gallery

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  • Western Blot (WB) analysis of hTERT-RPE1 cells using FOXC2 Monoclonal antibody (68384-1-Ig)

    WB analysis of hTERT-RPE1 using 68384-1-Ig (same clone as 68384-1-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOXC2 Monoclonal antibody (68384-1-Ig)

    WB analysis using 68384-1-Ig (same clone as 68384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOXC2 Monoclonal antibody (68384-1-Ig)

    IHC staining of human breast cancer using 68384-1-Ig (same clone as 68384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOXC2 Monoclonal antibody (68384-1-Ig)

    IHC staining of human breast cancer using 68384-1-Ig (same clone as 68384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

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FOXC2 Monoclonal antibody, PBS Only

FOXC2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68384-1-PBS
Clone No.3E3E1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Pig

Applications

WB, IHC, ELISA

Synonyms

FKHL14, Forkhead box protein C2, FOXC2, LD, Mesenchyme fork head protein 1, MFH 1, MFH 1 protein, MFH1, Transcription factor FKH 14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68384-1-PBS targets FOXC2 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Pig samples.

Tested Reactivity Human, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FOXC2 fusion protein Ag19378 相同性解析による交差性が予測される生物種
Full Name forkhead box C2 (MFH-1, mesenchyme forkhead 1)
Calculated molecular weight 501 aa, 54 kDa
Observed molecular weight56 kDa
GenBank accession numberBC113437
Gene Symbol FOXC2
Gene ID (NCBI) 2303
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99958
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708)