FOXC2 Recombinant antibody, PBS Only (Detector)

Sandwich ELISA standard curve of MP00470-4, Human FOXC2 Recombinant Matched Antibody Pair - PBS only. 83476-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33121. 83476-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

The mean FOXC2 concentration was determined to be 2.8 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

Immunofluorescent analysis of (4% PFA) fixed A375 cells using FOXC2 antibody (83476-5-RR, Clone: 240403H7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-5-RR, Clone:240403H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-5-RR, Clone:240403H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 83476-5-RR against Human FOXC2 were performed. The affinity constant is 2.79 nM.