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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXH1-Specific Polyclonal antibody

FOXH1-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20268-1-AP

Synonyms

FAST 1, Fast 2, FAST1, FAST2, forkhead box H1, Forkhead box protein H1, FOXH1, hFAST 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using FOXH1-Specific Polyclonal antibody (20268-1-AP)

    WB analysis of mouse liver using 20268-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20268-1-AP (FOXH1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using FOXH1-Specific Polyclonal antibody (20268-1-AP)

    WB analysis of L02 using 20268-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20268-1-AP (FOXH1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse liver tissue, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20268-1-AP targets FOXH1-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name forkhead box H1
Calculated molecular weight 39 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberNM_003923
Gene symbol FOXH1
Gene ID (NCBI) 8928
RRIDAB_10694676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXH1, also named as FAST1 and FAST2, is a transcriptional activator. FOXH1 recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. It is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. It forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). The antibody is specific to FOXH1.

Protocols

Product Specific Protocols
WB protocol for FOXH1-Specific antibody 20268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols