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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXK1 Polyclonal antibody (29338-1-AP)

    WB analysis using 29338-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29338-1-AP (FOXK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using FOXK1 Polyclonal antibody (29338-1-AP)

    IP experiment of K-562 using 29338-1-AP

    IP result of anti-FOXK1 (IP:29338-1-AP, 4ug; Detection:29338-1-AP 1:3000) with K-562 cells lysate 1800 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FOXK1 Polyclonal antibody (29338-1-AP)

    IHC staining of human colon cancer using 29338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FOXK1 Polyclonal antibody (29338-1-AP)

    IHC staining of human colon cancer using 29338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SW480 cells using FOXK1 Polyclonal antibody (29338-1-AP)

    IF Staining of SW480 using 29338-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using FOXK1 antibody (29338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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FOXK1 Polyclonal antibody

FOXK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 29338-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

FOXK1L, MNF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MDA-MB-231 cells, MKN-45 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSW480 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29338-1-AP targets FOXK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXK1 fusion protein Ag28820 相同性解析による交差性が予測される生物種
Full Name forkhead box K1
Calculated molecular weight 75 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberNM_001037165
Gene Symbol FOXK1
Gene ID (NCBI) 221937
RRIDAB_3669682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP85037
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Forkhead box (FOX) domains bind DNA and consist of 2 loops connecting beta-sheets that flank an alpha-helix, which is a group of highly conserved transcription factors. Forkhead box k1 (FOXK1) also known as MNF, is a transcription factor that belongs to the forkhead family consisting of the winged-helix DNA-binding domain and the N-terminal and C-terminal transcriptional domains(PMID: 15289879, PMID: 27223064). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. FoxK1-α (90 kDa) is expressed in committed myoblasts and differentiated myotubes, while FoxK1-β (55 kDa) is expressed mainly in quiescent satellite cells(PMID: 9271401).

Protocols

Product Specific Protocols
WB protocol for FOXK1 antibody 29338-1-APDownload protocol
IHC protocol for FOXK1 antibody 29338-1-APDownload protocol
IF protocol for FOXK1 antibody 29338-1-APDownload protocol
IP protocol for FOXK1 antibody 29338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols