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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXK1 Polyclonal antibody (29338-1-AP)

    WB analysis using 29338-1-AP (same clone as 29338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29338-1-AP (FOXK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using FOXK1 Polyclonal antibody (29338-1-AP)

    IP experiment of K-562 using 29338-1-AP (same clone as 29338-1-PBS)

    IP result of anti-FOXK1 (IP:29338-1-AP, 4ug; Detection:29338-1-AP 1:3000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FOXK1 Polyclonal antibody (29338-1-AP)

    IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FOXK1 Polyclonal antibody (29338-1-AP)

    IHC staining of human colon cancer using 29338-1-AP (same clone as 29338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SW480 cells using FOXK1 Polyclonal antibody (29338-1-AP)

    IF Staining of SW480 using 29338-1-AP (same clone as 29338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using FOXK1 antibody (29338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29338-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29338-1-PBS targets FOXK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXK1 fusion protein Ag28820 相同性解析による交差性が予測される生物種
Full Name forkhead box K1
Calculated molecular weight 75 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberNM_001037165
Gene Symbol FOXK1
Gene ID (NCBI) 221937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP85037
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Forkhead box (FOX) domains bind DNA and consist of 2 loops connecting beta-sheets that flank an alpha-helix, which is a group of highly conserved transcription factors. Forkhead box k1 (FOXK1) also known as MNF, is a transcription factor that belongs to the forkhead family consisting of the winged-helix DNA-binding domain and the N-terminal and C-terminal transcriptional domains(PMID: 15289879, PMID: 27223064). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. FoxK1-α (90 kDa) is expressed in committed myoblasts and differentiated myotubes, while FoxK1-β (55 kDa) is expressed mainly in quiescent satellite cells(PMID: 9271401).