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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXN2 Recombinant antibody, PBS Only (Capture)

FOXN2 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240896C8

Cat no : 83899-2-PBS

Synonyms

Human T-cell leukemia virus enhancer factor, HTLF, Forkhead box protein N2, forkhead box N2, 240896C8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FOXN2 Recombinant antibody (83899-2-RR)

    WB analysis using 83899-2-RR (same clone as 83899-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83899-2-RR (FOXN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83899-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00842-3

    Cytometric bead array standard curve of MP00842-3

    Cytometric bead array standard curve of MP00842-3, FOXN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83899-2-PBS. Detection antibody: 83899-4-PBS. Standard: Ag5126. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 83899-2-RR

    Affinity and Kinetic Characterization of 83899-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83899-2-RR against Human FOXN2 were performed. The affinity constant is 2.90 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83899-2-PBS targets FOXN2 as part of a matched antibody pair:

MP00842-3: 83899-2-PBS capture and 83899-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXN2 fusion protein Ag5126 相同性解析による交差性が予測される生物種
Full Name forkhead box N2
Calculated molecular weight 47 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC063305
Gene symbol FOXN2
Gene ID (NCBI) 3344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Forkhead box N2 (FOXN2), a member of the FOX transcription factor family, has been found to play key roles in various types of human cancers. A recent report has indicated that FOXN2 is downregulated and serves as a tumor suppressor in T-cell leukemia. Moreover, downregulation of FOXN2 is closely associated with adverse prognosis in adult glioblastoma multiforme treated with radiotherapy or chemotherapy. (PMID: 30655703)