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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXN3 Polyclonal antibody (25399-1-AP)

    WB analysis using 25399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25399-1-AP (FOXN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FOXN3 Polyclonal antibody (25399-1-AP)

    IF Staining of U2OS using 25399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FOXN3 antibody (25399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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FOXN3 Polyclonal antibody

FOXN3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25399-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C14orf116, Checkpoint suppressor 1, CHES1, forkhead box N3, Forkhead box protein N3, FOXN3, PRO1635

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, mouse embryo tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25399-1-AP targets FOXN3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXN3 fusion protein Ag21929 相同性解析による交差性が予測される生物種
Full Name forkhead box N3
Calculated molecular weight 490 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC007506
Gene Symbol FOXN3
Gene ID (NCBI) 1112
RRIDAB_3085795
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00409
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

As transcriptional regulators, the FOX protein family regulates the development of tissues, embryos as well as carbohydrate and lipid metabolism. Forkhead box N3 (FOXN3), also known as checkpoint suppressor 1 (CHES1), is initially identified from yeast and belongs to the FOX protein family as it contains a conserved FOX DNA binding domain in its N-terminal(PMID: 36450706). FOXN3 had been dysregulated in many types of malignancies including melanoma, osteosarcoma, hepatocellular carcinoma and breast cancer, and its expression level is strongly associated with progression and prognosis of patients(PMID: 31214487).

Protocols

Product Specific Protocols
WB protocol for FOXN3 antibody 25399-1-APDownload protocol
IF protocol for FOXN3 antibody 25399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols