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  • Featured Product
  • KD/KO Validated

FOXO1 Polyclonal antibody, PBS Only

FOXO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18592-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXO1 Polyclonal antibody (18592-1-AP)

    WB analysis using 18592-1-AP (same clone as 18592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using FOXO1 Polyclonal antibody (18592-1-AP)

    IP experiment of HepG2 using 18592-1-AP (same clone as 18592-1-PBS)

    IP result of anti-FOXO1 (IP:18592-1-AP, 4ug; Detection:18592-1-AP 1:2000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FOXO1 Polyclonal antibody (18592-1-AP)

    IHC staining of human kidney using 18592-1-AP (same clone as 18592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18592-1-AP (FOXO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FOXO1 Polyclonal antibody (18592-1-AP)

    IF Staining of HeLa using 18592-1-AP (same clone as 18592-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOXO1 antibody (18592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18592-1-PBS targets FOXO1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXO1 fusion protein Ag13296 相同性解析による交差性が予測される生物種
Full Name forkhead box O1
Calculated molecular weight 655 aa, 70 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC021981
Gene symbol FOXO1
Gene ID (NCBI) 2308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).