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  • Featured Product
  • KD/KO Validated

FOXO1 Monoclonal antibody, PBS Only

FOXO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D9A2

Cat no : 66457-1-PBS

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXO1 Monoclonal antibody (66457-1-Ig)

    WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FOXO1 Monoclonal antibody (66457-1-Ig)

    WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

    WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FOXO1 Monoclonal antibody (66457-1-Ig)

    WB analysis of HeLa using 66457-1-Ig (same clone as 66457-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using FOXO1 Monoclonal antibody (66457-1-Ig)

    WB analysis of SMMC-7721 using 66457-1-Ig (same clone as 66457-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66457-1-PBS targets FOXO1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOXO1 fusion protein Ag13296 相同性解析による交差性が予測される生物種
Full Name forkhead box O1
Calculated molecular weight 655 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC021981
Gene symbol FOXO1
Gene ID (NCBI) 2308
RRIDAB_2881826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).