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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXO1 Recombinant antibody, PBS Only (Capture)

FOXO1 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230183D1

Cat no : 82997-1-PBS

Synonyms

Forkhead box protein O1, forkhead box O1, FKHR, FKH1, FKH 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXO1 Recombinant antibody (82997-1-RR)

    WB analysis using 82997-1-RR (same clone as 82997-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82997-1-RR (FOXO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FOXO1 Recombinant antibody (82997-1-RR)

    IHC staining of human pancreas cancer using 82997-1-RR (same clone as 82997-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82997-1-RR (FOXO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FOXO1 Recombinant antibody (82997-1-RR)

    IF Staining of HepG2 using 82997-1-RR (same clone as 82997-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXO1 antibody (82997-1-RR, Clone: 230183D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00024-1

    Cytometric bead array standard curve of MP00024-1

    Cytometric bead array standard curve ofMP00024-1, FOXO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82997-1-PBS. Detection antibody: 82997-3-PBS. Standard: Ag13296. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 82997-1-RR

    Affinity and Kinetic Characterization of 82997-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82997-1-RR against Human FOXO1 were performed. The affinity constant is 0.188 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82997-1-PBS targets FOXO1 as part of a matched antibody pair:

MP00024-1: 82997-1-PBS capture and 82997-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXO1 fusion protein Ag13296 相同性解析による交差性が予測される生物種
Full Name forkhead box O1
Calculated molecular weight 655 aa, 70 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC021981
Gene symbol FOXO1
Gene ID (NCBI) 2308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).