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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXO1 Recombinant antibody, PBS Only (Capture)

FOXO1 Recombinant Antibody for WB, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230183D1

Cat no : 82997-1-PBS

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXO1 Recombinant antibody (82997-1-RR)

    WB analysis using 82997-1-RR (same clone as 82997-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82997-1-RR (FOXO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FOXO1 Recombinant antibody (82997-1-RR)

    IHC staining of human pancreas cancer using 82997-1-RR (same clone as 82997-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82997-1-RR (FOXO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00024-1

    Cytometric bead array standard curve of MP00024-1

    Cytometric bead array standard curve ofMP00024-1, FOXO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82997-1-PBS. Detection antibody: 82997-3-PBS. Standard: Ag13296. Range: 0.625-20 ng/mL.

  • Affinity experiments of 82997-1-RR

    Affinity experiments of 82997-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82997-1-RR against Human FOXO1 were performed. The affinity constant is 0.188 nM.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82997-1-PBS targets FOXO1 as part of a matched antibody pair:

MP00024-1: 82997-1-PBS capture and 82997-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXO1 fusion protein Ag13296 相同性解析による交差性が予測される生物種
Full Name forkhead box O1
Calculated molecular weight 655 aa, 70 kDa
GenBank accession numberBC021981
Gene symbol FOXO1
Gene ID (NCBI) 2308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.