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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXO3A Monoclonal antibody (66428-1-Ig)

    WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOXO3A Monoclonal antibody (66428-1-Ig)

    WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FOXO3A Monoclonal antibody (66428-1-Ig)

    WB analysis of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

    WB result of FOXO3A antibody (66428-1-Ig, 1:12,000) with si-Control and si-FOXO3A transfected HEK293 cells. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOXO3A Monoclonal antibody (66428-1-Ig)

    WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using FOXO3A Monoclonal antibody (66428-1-Ig)

    IP experiment of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

    IP result of anti-FOXO3A (IP:66428-1-Ig, 4ug; Detection:66428-1-Ig 1:1000) with HEK-293 cells lysate 2200 ug. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human breast cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human lung cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human lung cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human ovary tumor using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human ovary tumor using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human stomach cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66428-1-Ig (FOXO3A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human stomach cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66428-1-Ig (FOXO3A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOXO3A Monoclonal antibody (66428-1-Ig)

    IHC staining of human breast cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FOXO3A Monoclonal antibody (66428-1-Ig)

    IF Staining of HepG2 using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66428-1-Ig(FOXO3A antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated Goat Anti-mouse IgG(H+L). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FOXO3A Monoclonal antibody, PBS Only

FOXO3A Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66428-1-PBS
Clone No.1F12D11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66428-1-PBS targets FOXO3A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FOXO3A fusion protein Ag1289 相同性解析による交差性が予測される生物種
Full Name forkhead box O3
Calculated molecular weight 673 aa, 71 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC021224
Gene Symbol FOXO3A
Gene ID (NCBI) 2309
RRIDAB_2881799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43524
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)