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- KD/KO Validated
FOXO6 Polyclonal antibody
FOXO6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 19122-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C2C12 cells, mouse brain tissue, mouse lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
19122-1-AP targets FOXO6 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | forkhead box protein O6 |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | AI361654 |
| Gene symbol | FOXO6 |
| Gene ID (NCBI) | 100132074 |
| RRID | AB_10597247 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has an isoform of 70 kDa when modified after translation (PMID:23639108).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| IHC protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| IF protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| IP protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging Cell Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling | ||
Elife Inhibition of mutant EGFR in lung cancer cells triggers SOX2-FOXO6-dependent survival pathways.
| ||
RNA Biol Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.
| ||
Oncotarget Involvement of the FOXO6 transcriptional factor in breast carcinogenesis.
| ||
Oncotarget Elevated FOXO6 expression correlates with progression and prognosis in gastric cancer. | ||
Mol Cell Biol Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-dependent Manner. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Young-Wook (Verified Customer) (10-31-2017) | This antibody detect easily mouse Foxo1 than mouse Foxo6. There is cross reactivity with Foxo1.
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