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  • KD/KO Validated

FOXO6 Polyclonal antibody

FOXO6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 19122-1-AP

Synonyms

forkhead box protein O6


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Tested Applications

Positive WB detected inC2C12 cells, mouse brain tissue, mouse lung tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19122-1-AP targets FOXO6 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name forkhead box protein O6
Calculated molecular weight 51 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberAI361654
Gene symbol FOXO6
Gene ID (NCBI) 100132074
RRIDAB_10597247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has an isoform of 70 kDa when modified after translation (PMID:23639108).

Protocols

Product Specific Protocols
WB protocol for FOXO6 antibody 19122-1-APDownload protocol
IHC protocol for FOXO6 antibody 19122-1-APDownload protocol
IF protocol for FOXO6 antibody 19122-1-APDownload protocol
IP protocol for FOXO6 antibody 19122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging Cell

Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling

Authors - Ruili Dang
humanWB, IF

Elife

Inhibition of mutant EGFR in lung cancer cells triggers SOX2-FOXO6-dependent survival pathways.

Authors - S Michael Rothenberg
  • KD Validated
pigWB,ChIP,CoIP

RNA Biol

Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.

Authors - Xin Zong
  • KD Validated
humanWB

Oncotarget

Involvement of the FOXO6 transcriptional factor in breast carcinogenesis.

Authors - François Lallemand
  • KD Validated
humanIHC

Oncotarget

Elevated FOXO6 expression correlates with progression and prognosis in gastric cancer.

Authors - Jia-Hong Wang
humanIF

Mol Cell Biol

Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-dependent Manner.

Authors - Megumi Ikeda

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Young-Wook (Verified Customer) (10-31-2017)

This antibody detect easily mouse Foxo1 than mouse Foxo6. There is cross reactivity with Foxo1.

  • Applications: Western Blot
  • Primary Antibody Dilution: 2000
  • Cell Tissue Type: 293T overexpression
FOXO6 Antibody Western Blot validation (2000 dilution) in 293T overexpression (Cat no:19122-1-AP)