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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXP1 Polyclonal antibody

FOXP1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 22051-1-AP

Synonyms

Mac-1-regulated forkhead, HSPC215, hFKH1B, Forkhead box protein P1, forkhead box P1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of Jurkat using 22051-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of A549 using 22051-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of mouse testis using 22051-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of PC-3 using 22051-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of MDA-MB-453s using 22051-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of Y79 using 22051-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of mouse lung using 22051-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of Raji using 22051-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of LNCaP using 22051-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of MCF-7 using 22051-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FOXP1 Polyclonal antibody (22051-1-AP)

    WB analysis of mouse heart using 22051-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using FOXP1 Polyclonal antibody (22051-1-AP)

    IHC staining of mouse embryo using 22051-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using FOXP1 Polyclonal antibody (22051-1-AP)

    IHC staining of mouse embryo using 22051-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Jurkat cells using FOXP1 Polyclonal antibody (22051-1-AP)

    FC experiment of Jurkat using 22051-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FOXP1 (22051-1-AP) and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inJurkat cells, A549 cells, LNCaP cells, MCF-7 cells, MDA-MB-453s cells, mouse heart tissue, mouse lung tissue, mouse testis tissue, PC-3 cells, Raji cells, Y79 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

22051-1-AP targets FOXP1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXP1 fusion protein Ag17045 相同性解析による交差性が予測される生物種
Full Name forkhead box P1
Calculated molecular weight 677 aa, 75 kDa
Observed molecular weight 50 kDa, 60-65 kDa, 85 kDa
GenBank accession numberBC131720
Gene symbol FOXP1
Gene ID (NCBI) 27086
RRIDAB_2878980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP1, also known as Mac-1-regulated forkhead, is a 677 amino acid protein, which forms homodimers and heterodimers with FOXP2 and FOXP4 (PubMed:25027557). Dimerization is required for DNA-binding. FOXP1 has an important function in neuronal development.9 Mutations of its gene, FOXP1, located on chromosome 3p14.1,7 can result in the development of autism spectrum disorder, intellectual disability, speech and language deficits as well as motor development delay. FOXP1 is also engaged in lung and esophagus morphogenesis, as well as in B-cell development.7,10 The widely researched role of FOXP1 in carcinogenesis is of great importance, although still unclear to some extent. FOXP1 exists some isoforms with MV 75-77 kDa, 65-67 kDa, 12 kDa.

Protocols

Product Specific Protocols
WB protocol for FOXP1 antibody 22051-1-APDownload protocol
IHC protocol for FOXP1 antibody 22051-1-APDownload protocol
FC protocol for FOXP1 antibody 22051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int Immunopharmacol

Long non-coding RNA NEAT1 exacerbates NLRP3-mediated pyroptosis in allergic rhinitis through regulating the PTBP1/FOXP1 cascade

Authors - Yunliang Liu
View Article
humanWB

J Cell Mol Med

LINC01116 promotes proliferation and migration of endometrial stromal cells by targeting FOXP1 via sponging miR-9-5p in endometriosis.

Authors - Liangyi Cui
View Article