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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of HEK-293 using 22173-1-AP

    WB result of FOXP3 antibody (22173-1-AP, 1:500) with si-Control and si-FOXP3 transfected HEK293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of HEK-293 using 22173-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of RAW264.7 using 22173-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of RAW264.7 using 22173-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of HeLa using 22173-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of HeLa using 22173-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of RAW264.7 using 22173-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of HEK-293 using 22173-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of HEK-293 using 22173-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of A375 using 22173-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FOXP3 Polyclonal antibody (22173-1-AP)

    WB analysis of HEK-293 using 22173-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for WB, ELISA
Cat No. 22173-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HEK-293

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22173-1-AP targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXP3 fusion protein Ag17827 相同性解析による交差性が予測される生物種
Full Name forkhead box P3
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC113401
Gene Symbol FOXP3
Gene ID (NCBI) 50943
RRIDAB_2879017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZS1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Protocols

Product Specific Protocols
WB protocol for FOXP3 antibody 22173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Ther Med

Poly(ADP-ribose) polymerase-1 inhibitor ameliorates dextran sulfate sodium-induced colitis in mice by regulating the balance of Th17/Treg cells and inhibiting the NF-κB signaling pathway.

Authors - Shuai Peng
View Article
humanIHC

Oral Dis

Influence of PD-1/PD-L1 on immune micro-environment in oral leukoplakia and oral squamous cell carcinomas

Authors - Qiong Wang
View Article