FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 22228-1-AP

Synonyms

Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER


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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22228-1-AP targets FOXP3 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXP3 fusion protein Ag17571 相同性解析による交差性が予測される生物種
Full Name forkhead box P3
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC113401
Gene symbol FOXP3
Gene ID (NCBI) 50943
RRIDAB_11182376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Protocols

Product Specific Protocols
WB protocol for FOXP3 antibody 22228-1-APDownload protocol
IHC protocol for FOXP3 antibody 22228-1-APDownload protocol
IF protocol for FOXP3 antibody 22228-1-APDownload protocol
IP protocol for FOXP3 antibody 22228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Acta Pharm Sin B

Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy

Authors - Hao Ren
mouse

Cancer Res

Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.

Authors - Binghao Li
mouse

Biomaterials

LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.

Authors - Feng Liu
humanWB

Clin Transl Med

MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.

Authors - Yuanyuan Chang
humanWB

J Autoimmun

NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.

Authors - Mengxiao Xie
humanWB

Biomed Pharmacother

Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis

Authors - Qiuyan Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anne-Laure (Verified Customer) (12-07-2021)

non-specific staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: brain slices from mouse