FOXP3 Polyclonal antibody
FOXP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 22228-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 42 publications below |
IHC | See 1 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
FC | See 2 publications below |
CoIP | See 1 publications below |
ChIP | See 2 publications below |
Product Information
22228-1-AP targets FOXP3 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FOXP3 fusion protein Ag17571 相同性解析による交差性が予測される生物種 |
Full Name | forkhead box P3 |
Calculated molecular weight | 431 aa, 47 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC113401 |
Gene symbol | FOXP3 |
Gene ID (NCBI) | 50943 |
RRID | AB_11182376 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.
Protocols
Product Specific Protocols | |
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WB protocol for FOXP3 antibody 22228-1-AP | Download protocol |
IHC protocol for FOXP3 antibody 22228-1-AP | Download protocol |
IF protocol for FOXP3 antibody 22228-1-AP | Download protocol |
IP protocol for FOXP3 antibody 22228-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Pharm Sin B Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy | ||
Cancer Res Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma. | ||
Biomaterials LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy. | ||
Clin Transl Med MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis. | ||
Biomed Pharmacother Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anne-Laure (Verified Customer) (12-07-2021) | non-specific staining
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