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Validation Data Gallery

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  • Western Blot (WB) analysis of MJ cells using FOXP3 Monoclonal antibody (68803-2-Ig)

    WB analysis of MJ using 68803-2-Ig

    MJ cells were subjected to SDS PAGE followed by western blot with 68803-2-Ig (FOXP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human tonsillitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human ovary cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human ovary cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human appendicitis using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of MJ cells using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of MJ using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of MJ cells using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of MJ using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded MJ cells slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human lymphoma using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using FOXP3 Monoclonal antibody (68803-2-Ig)

    IHC staining of human rectal cancer using 68803-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68803-2-Ig (FOXP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FOXP3 Monoclonal antibody

FOXP3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68803-2-Ig
Clone No.2A1E7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER, AIID

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMJ cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human lymphoma tissue, human ovary cancer tissue, human rectal cancer tissue, MJ cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68803-2-Ig targets FOXP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOXP3 fusion protein Ag23089 相同性解析による交差性が予測される生物種
Full Name forkhead box P3
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC113401
Gene Symbol FOXP3
Gene ID (NCBI) 50943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BZS1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP3 is a transcription factor belonging to the Forkhead box (FOX) protein family. It contains a forkhead-winged helix domain that acts as a transcriptional activator or repressor. FOXP3 is the master regulator of regulatory T cells (Tregs), a subset of CD4+ T cells with potent suppressive activity. It plays a critical role in maintaining self-tolerance and preventing autoimmune diseases by controlling the development and function of Tregs. The expression of FOXP3 is quite limited, and the cellular taxa present in the tissue are relatively low. Therefore, if you intend to perform Western blotting (WB), it is advisable to enrich the target cells prior to detection.

Protocols

Product Specific Protocols
WB protocol for FOXP3 antibody 68803-2-IgDownload protocol
IHC protocol for FOXP3 antibody 68803-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols